Search results for "Islets transplantation"

showing 3 items of 3 documents

A new method to valued efficiency of enzyme blend for pancreas tissue digestion.

2009

One of the best successful example of cell therapy is represented by islet transplantation since the '90. However islet isolation methods are not completely standardized yet. More than half of isolation procedures failed to isolate adequate islets for transplantation, due to variable pancreas condition, and to unpredictable enzymatic blend efficiency. Enzymes used for pancreas digestion are purified from Clostridium histolyticum; these enzymes has a broad substrate specificity and potent collagenolytic activity compared to vertebrate collagenases. However, a major obstacle in human islet isolation successful is due to the variability in composition and concentration of the collagenases used…

Islets transplantationSettore BIO/10 - BiochimicaCollagenaseTissue digestionLangerhan
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Standardization of islet isolation outcome- A new automatic system to determine pancreatic islet viability

2011

Pancreatic islet transplantation is emerging as a therapeutic approach for patients affected by diabetes. This approach consists of a minimally invasive procedure replacing insulin-producing cells (pancreatic islets). The technique has been proven successful, but limitations have been identified. One of the major challenges of the procedure is the counting of the isolated pancreatic islets, which is currently jeopardized by subjectivity and inaccuracy. Determination of the accurate islet number is a crucial factor in determining the correlation between the isolation product and clinical outcome. In the proposed study, we have developed software capable of objectively evaluating islet number…

endocrine systemgeographygeography.geographical_feature_categoryIsolation (health care)Standardizationbusiness.industryPancreatic isletsGeneral EngineeringArea of interestIsletBioinformaticsOutcome (game theory)Computer Science Applicationsmedicine.anatomical_structureArtificial IntelligenceMedicinePancreatic islet transplantationImage analysis feature extraction pancreatic islets transplantationbusinessMinimally invasive procedures
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A new method to value efficiency of enzyme blends for pancreatic tissue digestion.

2010

Islet transplantation, since the 90’s, has been resulting to be one of the best successful example of human cell therapy. Nevertheless, islet isolation procedure is not completely standardized; in fact, more than fifty percent of islets procedures don’t arrive to their transplantation. This is due both to the variability of donor’s pancreas and to an unpredictable enzymatic blend efficiency. Enzymes used in pancreas digestion are extracted from Clostridium histolyticum bacteria and digest several substrates. In particular they have strong collagenolytic activity compared to vertebrate collagenases. However, several impediments persist in human islet isolation success probably due to the var…

endocrine systemmedicine.medical_specialtyProteasesIslets transplantationmedicine.medical_treatmentCollagenaseIslets of Langerhans TransplantationThermolysinCell SeparationCell LineIslets of LangerhansClostridium histolyticumSettore BIO/10 - BiochimicaInternal medicinemedicineHumansCollagenasesPancreasTransplantationIslet cell transplantationgeographyEvaluation alive cellgeography.geographical_feature_categorybiologyPancreatic isletsREcombinant proteinProteolytic enzymesEndothelial Cellsproteolytic enzymesbiology.organism_classificationIsletTransplantationmedicine.anatomical_structureEndocrinologyBiochemistryGelatinasesSurgeryCollagenPancreasGelsPeptide HydrolasesTransplantation proceedings
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